Study of the effect of berberine on the efficacy of radiation toward A549 tumors

• Main Authors: Ching-Ming, 洪晴敏
• Other Authors: 周芬碧
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/06753227505981460891

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 100 === The current conventional cancer treatments are divided into chemotherapy and radiation treatment; chemical treatment known as the most effective cancer treatment the use of one or more of the drugs in combination (also known as combined chemotherapy), and its role in destruction of cancer cells, to cure cancer, control the disease, relieve symptoms purposes. Addition of chemotherapy to kill cancer cells, but also for normal cell growth and more rapid damage and side effects. The use of radiation therapy that is high energy radiation, is about the diagnostic X-ray energy of hundreds or thousands of times, to destroy cancer cells, prevent cancer cell growth and division, is currently the main strategy of the treatment of lung cancer one. But also a dose of normal tissue tolerance limits, and radiation for different cells have different sensitivity, and radiation for cancer patients also have a considerable degree of side effects; Purpose of this study is the use of this herbal medicine berberine to radiation therapy combined with a view to assist in lung cancer cells and tumor cytotoxic, or even to reduce the radiation dose to reduce side effects of cancer patients. In animal experiments, using nude mice in the subcutaneous growing human lung cancer cells A549, found that aging rats given berberine combined radiation will significantly inhibit tumor size, and then to Western blot to analyze protein expression, to explore its role in the mechanism of, the experimental results Bcl-2, caspase-3, ATG-5 and LC-3 protein expression level display, use of berberine combined radiation experiments in apotosis and autophage, no significant correlation.