Determination of common color additives in cosmetics by liquid chromatography

• Main Authors: Jung Su, 蘇瑢
• Other Authors: Yuan-Po Lee
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/394jnq

碩士 === 嘉南藥理科技大學 === 化妝品科技研究所 === 100 === The regulations of colorants in cosmetics are different from country to country. The manufacture have to aware the regulations in different countries and also avoid the cross contamination during manfacturing process. Therefore, a suitable analytical method will play an important role in the process quality control of cosmetics. Thin-layer chromatography and ion-pair liquid chromatography are used in this study to analyze nine most common used color additives in cosmetics. The result show that the solubilities of some colorants from different resources exhibit. Obvious differences and hence increase the complexity of pretreatment. The results of thin-layer chromatography show better resolution when methanol is used as solvent and eluent. However, there are also some colorants could not be separated which probably due to the formation of some molecular during pretreatment process. The deserves further study. The results of ion-pair liquid chromatography show slower retention time of colorants as the ratio of ion-pair reagent in eluent increased. Better resolution is achieved when 2.2mM tetramethyl ammonium hydroxide was used as ion-pair reagent. The best resolution was achieved with gradient elution which was also applied to the analysis of commercial products. It could be an important reference for colorant analysis of cosmetics.