Studies on the antioxidative constituents from the leaves ofSolanum lasiocarpum

• Main Authors: Yu-shu Lin, 林煜書
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/fys3gq

碩士 === 嘉南藥理科技大學 === 藥物科技研究所 === 100 === Solanum lasiocarpum Dunal (SL, Solanaceae) has been used as a folk medicine in Taiwan to treat cough, sore throat, edema, bruises, and hernia. There is no report about the microscopic tissues identification and pharmacological activities of the SL. The aim of this study was focused on the roots, stems and leaves to establish the microscopic tissues identification spectrum and isolate the antioxidative constituents from SL according to the activity-guided isolation process. DPPH radical scavenging ability, Trolox equivalent antioxidant capacity and total phenol contents were applied as screening platforms of antioxidation. The SL was collected in Taiwan and divided into four parts, including roots, stems, leaves and fruit. Four parts of SL were respectively extracted with H2O and 95 % EtOH to obtain total eight crude extracts of SL. Eight crude extracts were evaluated by screening platforms of antioxidation, and the results showed that the most potent antioxidative extract was 95 %EtOH extract of leaves of SL (SLLE). A large amount (1.1 Kg) of leaves of SL was extracted with 95 % EtOH and obtained 116.53 g of SLLE extract. The SLLE was partitioned with H2O, CH2Cl2, EtOAc, and n-BuOH solvents to obtain six extracts, including CH2Cl2 layer extract (SLLED), Insoluble layer extract (SLLEI), EtOAc layer extract (SLLEA), Emulsion layer extract (SLLEE), n-BuOH layer extract (SLLEB) and H2O layer extract (SLLEW), respectively. The six layer extracts were evaluated by screening platforms of antioxidation, and the results showed that the most potent antioxidative layer extract was SLLEA. We selected SLLEA to isolate the antioxidative constituents with chromatography and screening platforms of antioxidation methods. SLLEA extract was isolated by silica gel column chromatography and TLC. The results showed that the most potent antioxidative fraction was SLLEA2-2 and SLLEA2-3. Paprazine was identified from SLLEA2-3. The active constituents of SLLEA2-2 will further be isolated and identified their structures. We also obtained a mixture of salt of Iron (II) and Cupric sulfate.