Exploration of Rath cell-penetrating peptide in intracellular delivery

• Main Authors: Chia-wei Lin, 林佳緯
• Other Authors: Jung-hua Kuo
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/mysa73

碩士 === 嘉南藥理科技大學 === 藥物科技研究所 === 100 === Interest in using cell-penetrating peptides (CPPs) or protein transduction domains as carriers for intracellular delivery is increasing. Cell-penetrating peptides, alone or coupled with various cargo molecules, can cross biological membrane barriers without significantly damaging the membranes and with little cytotoxicity to the cells. Of the cell-penetrating peptides used, Rath peptide, derived from the avian infectious bursal disease virus, has been identified. Rath peptide is capable of non-covalent interaction and delivering large cargo to primary cells. Therefore, the aim of this study is to characterize and explore of Rath peptide in intracellular delivery. Biocompatibility of Rath peptide and its conjugation of FITC (fluorescein-5-isothiocynate) were tested and the optimal dose ranges were determined. Also, Cell uptake of Rath conjugated FITC in N-terminus was higher than that in C-terminus. We found that Rath peptide is capable of delivering FITC to U-937 and HeLa cells.