| Summary: | 碩士 === 嘉南藥理科技大學 === 溫泉產業研究所 === 100 === Abstract
Explore the effects of hot springs on the melanin formatted by hydroxyl radical. The research references had shown that the ultraviolet and the free radical were caused the skin pigmentation and the carbonate and sulfuric acid spring water hand down the skin beauty and whitening effect, however, there were only few evidences to know the whitening mechanisms of hot springs. Our preliminary research had shown the hydrogen carbonatic spring to have the absorption hydroxyl free radical effect. Furthermore, there are different mechanisms to increase pigmentation and inhibit pigment decomposition between the carbonate and sulfuric acid spring water. In this study we will discuss the possible mechanism of spring water on pigmentation, and specific focus on the hydroxyl radical activity. Basic on this study, compared hot springs (Beitou, Taian, Guanziling, Polaris, Szuchung, Antong, Chihpen) with the NaCl solution (control group), the pigmentation was induced by Fenton reaction to produce hydroxyl free radical, which reactive the tyrosine format to melanin. After the Fenton reaction, our data have shown that absorbance (450 nm) of control group on pigmentation at the time 10 min was 0.115±0.026, 30 min was 0.111±0.018, then still stable on plateaus to 60 min was 0.108±0.017. Compared control group to hot springs, the absorbance of hot springs on pigmentation at the time 10, 30 and 60 min each were Beitou (0.297±0.028, 0.301±0.026 and 0.305±0.022), Taian (0.297±0.039, 0.288±0.021 and 0.285±0.020), Guanziling (0.376±0.031,0.373±0.026and 0.369±0.025), Polaris (0.234±0.030, 0.237±0.029 and 0.241±0.029), Szuchung (0.243±0.025, 0.247±0.025 and 0.250±0.023), Chihpen (0.201±0.018, 0.203±0.019 and 0.205±0.019) and Antong (0.111±0.020, 0.113±0.020 and 0.116±0.020). At the time 10,30 and 60 min, all of hot spring groups and control group comparison were shown a statistically significant difference (p<0.001), except Antong. Specially, the Guanziling hot spring produced a powerful enhanced effect than the others.
This finding has suggested the possible mechanisms of whitening effect of hot springs on pigmentation that may be through reactive with hydroxyl free radical.
Keywords: Hot springs, Hydroxyl free radicals, Melanin, Whitening
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