Effects of Pennisetum purpureum on xenobiotic metabolism system and related oxidative stress in mice with ethanol-induced acute liver injury

• Main Authors: Po-Hsiang Huang, 黃勃翔
• Other Authors: Huey-Mei Shaw
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/49m8kh

碩士 === 嘉南藥理科技大學 === 保健營養系 === 100 === Background and Purpose: Pennisetum purpureum is commnly known as grass. Purple Pennisetum is a new species and has been named as Pennisetum 5th by the Tainan National Livestock Research Institute. Pennisetum contains high levels of anthocyanins and polyphenols which owning antioxidant effect. Nowadays, liver disease is one of the top ten causes of death for people in Taiwan. The alcoholic hapatitis and viral hepatitis which is increasing in Taiwan may then develop into liver cancer. Therefore, prevention of liver disease and protection of liver against oxidative stress is an important issue for the public health. Oxidative stress produced during alcohol metabolism causes liver cell injury. In this study, we would like to explore the protection effects of Pennisetum on acute alcoholic liver injury in mice, and the liver detoxification enzyme system was also measured. Methods: In part 1, the antioxidant effect of water extract of Pennisetum were measured in vitro. The Trolox equivalent antioxidant capacity（TEAC）, scavenging effect on 2,2-Diphenyl -1-picrylhydrazyl radicals , reducing power , chelating effects on ferrous ions were included. In part 2, the animal model was used to investigate the protection effects of Pennisetum. In the second part, 25 seven-weeks old C57BL/6J male mice were divided into four groups as the following: the control group (CS, Control + Saline,), alcohol group (CA, Control + Alcohol), PS group (5% Pennisetum + Saline,) and PA group ( 5% Pennisetum + Alcohol). All groups were fed for four-weeks. The alcohol (60% ethanol, 10ml/Kg BW) or saline were tube fed once 12 hours before sacrifice. Results: In part 1, all the antioxidant indicators measured showed an increasing effects as the concentration of Pennisetum extract increased. In the second part, The plasma activities of GPT and LDH in CA group were significantly higher than in CS group. This suggested that acute liver injury was induced in mice tube fed with alcohol. The GPT activity of the PA group was slightly lower than the CA group, but showed no significant difference. There were no significant differences among the four groups in TBARS levels and the antioxidant enzyme activities of CAT, SOD and GST in liver. The GPX activity and GSH levels in liver showed significantly lower in PS group than the CS group, but no significant differences among the PA, CA and CS group. The liver GR activity has a significant increase in the PS group than the CA group. The concentrations of liver Vit. E in CA group, PS group and PA group decreased significantly than in the CS group. In the PS and PA group, liver CYP3A protein content were significantly decreased when compared to the CS group. No significant differences were found in the CYP2E1 protein content among the four groups. Conclusion: Pennisetum extract showed antioxidant ability in vitro test. In animal model,Pennisetum feeding decreased the GPX activity and GSH concentration in liver, but showed no increase in liver lipid oxidation. There was no obvious protection effect of Pennisetum on acute ethanol-induced liver injury in mice. The inhibition of CYP3A protein expression was found by Pennisetum feeding. The interaction between Vit.E and CYP3A needs further investigation.