| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 100 === Nervous necrosis virus (NNV) threatens larvae and juveniles hatchery production in Taiwan. This virus causes massive mortality in grouper juveniles by viral nervous necrosis disease (VNN) and result in considerable economic losses in grouper aquaculture. NNV contains two, single stranded, positive-sense RNA called RNA1 and RNA2. RNA1 encodes RNA dependent RNA polymerase, and RNA2 encodes viral coat protein. From the observation of nervous necrosis virus outbreak in the farm, we found that temperature change could affect virus infection ability. Therefore, we would like to investigate whether the virus replication rate is affected directly by the grouper size or incubation temperature. In our study, we collected VNN diseased orange-spotted grouper samples from several aquaculture farms near Tainan from year 2010 to 2012. We established NNV challenge model by intraperitoneal injection of NNV to healthy grouper. We also use Real-time PCR and histopathological methods to detect the amount of NNV present in various tissue sample of infected and healthy grouper. Finally, we hope to find the relationship between temperature and NNV infection to reduce the impact of NNV infection in grouper aquaculture industry. Results demonstrated that virus replicate slower in 20℃ and replicate faster in 28℃. In addition, grouper juveniles are more tolerance for virus than larvae.
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