The fermentation production of porcine circovirus type2 Capsid protein from E.coli

• Main Authors: Chia-hao Kang, 康家豪
• Other Authors: Chien-min Chiang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/9wf93u

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 100 === Porcine circovirus 2 (PCV2) is the main viral pathogen that causes the desease called powtweaning multisystemic wasting syndrome. Capsid protein (Cap) of PCV2 containing the epitopes to elicit immune reaction, is the main target of subunit vaccine. In this study, the high cell density fermentation to produce the Cap protein of PCV2 were performed. The Cap protein was successfully expressed in E. coli Rosetta 2 (DE3) as a SUMO-Cap fusion protein, and the fusion protein was purified to homogenity by cation exchange chromatography. Through the digestion by SUMO protease, followed by coloum chromatrogaphy, Cap protein can be purified. The high cell density fermentation, coupled with lactose induction, for the production of SUMO-Cap were performed in this study. The results indicated that 12X ZYP feeding medium, containing 0.42% glycerol and 0.16% lactose as inducer, showed the highest fusion protein production, with production level even higher than that using IPTG as inducer.