| Summary: | 碩士 === 中國醫藥大學 === 分子系統生物醫學研究所碩士班 === 100 === Caffeic Acid Phenethyl Ester (CAPE) is an active component of propolis, which is a resinous hive product collected by honeybees from various plant sources. CAPE exhibits anticancer, anti-inflammatory and immunomodulatory activities. Caffeic Acid Decyl Ester (CADE) is an artificial compound with structure similar to CAPE. To study the effect of CADE on tumor cells and macrophages, v-Src transformed murine fibroblasts (IV5) and RAW264.7 macrophages were chosen as our study materials. Here we reported that CADE suppressed proliferation and migration in IV5 cells, impaired motility in LPS-stimulated macrophages. CADE reduced the expression of Src and Eps8, suppressed the activity of Src, FAK and ERK in IV5 cells. Consistent with the participation of Src and FAK in macrophage migration, CADE significantly inhibited the enhancement of Src and increased activity of Src and FAK in LPS-treated macrophages. In contrast, the expression of myeloid Src relatives (i.e. Lyn, Fgr and Hck) was almost constant in the presence or absence of CADE in LPS-stimulated macrophages. These findings indicated that by decrement of the protein level of Src as well as its activation, CADE could effectively suppress the migratory ability of IV5 and LPS-exposed macrophages.
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