| Summary: | 碩士 === 中國醫藥大學 === 基礎醫學研究所碩士班 === 100 === Ganoderma (Lingzhi) is a popular medicinal mushroom used as a traditional Chinese medicine (TCM) in Asian countries over the past two millennia. Previous studies have shown that the main anticancer constituents of Ganoderma are polysaccharides and triterpenoids. However, medicinal activities of Ganoderma may vary significantly from one species to another, mainly due to differences in the active ingredients. Therefore, this thesis investigated whether the ethanol extract of Ganoderma tsugae (GTE) could cause the growth inhibition of human colorectal and ovarian cancer cells followed by unraveling its active ingredients inside? The GTE was dissolved in 50% ethanol and then partitioned with ethyl acetate (EA), n-butanol (n-BuOH) in turn to obtain EA, n-BuOH and water fractions, respectively. Based on the results of MTT assay, the active EA fraction was collected and further separated into 10 fractions by column chromatography. The biological activity of each fraction was determined by MTT assay. Among the 10 fractions, fraction G has the best growth inhibition effect on human colorectal and ovarian cancer cells. Finally, HPLC (high performance liquid chromatography), LC-MS/MS (liquid chromatography-mass spectrometry / mass spectrometry) and NMR (nuclear magnetic resonance) were used to confirm the chemical structure of the peak ingredient. In conclusion, the GTE can cause growth inhibition of human ovarian and colorectal cancer cells. However, the inhibiting effect of the GTE ingredient ganoderic acid C2 remains to be confirmed by further investigation.
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