In vitro and in vivo investigation of novel biodegradable nanofibrous membranes

• Main Authors: Dave W. Chen, 陳威志
• Other Authors: S. J. Liu
• Format: Others
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/58837404280620229913

博士 === 長庚大學 === 機械工程學系 === 100 === Bioabsorbable implants have become increasingly popular in diverse applications used for fixation of bony and ligamentous structures in orthopedic surgery. However, the use of bioabsorbable membranes in tendon repair surgery has been sparsely documented in the literature. Polylactide–polyglycolide (PLGA) is in the class of synthesized biodegradable and biocompatible copolymers from which resorbable sutures, resorbable surgical clips, and controlled-release implants are made. PLGA also falls within the class of copolymers that have been used for implantable and injectable controlled release, drug delivery systems. These copolymers have been approved for, and have a history of safe use in, humans. After being introduced into the body, PLGA material induces only a minimal inflammatory response and biodegrades through the hydrolysis of its ester linkages to yield biocompatible lactic and glycolic acids. Furthermore, the rate at which PLGA biodegrades is a function of the ratio of lactide to glycolide. Previous study introduced the advantage effect of electrospun PLGA/collagen nanofibrous membrane as early-stage wound dressing. The bioabsorbable membranes are no longer the material of the scaffold but are functionally active in responses in human fibroblasts, and will be very effective as the accelerators in the tissue repair process. We will initiate a two-step study. In the first step, we will focus on the upgrade of the nanofibrous membrane. In addition to the previous collagen, the broad-spectrum antibiotics will be added into the membrane. In the second step, we will arrange the animal study for the investigation of tendon repair. With two different augmentation methods, the histology examination of the repaired site and the biomechanical testing of the tendon are investigated and are compared with those without augmentation technique.