Potassium Ion Sensing Improvements by Fluorine Doping and Organic Ionophore for Inflammasome Cell Detection

• Main Authors: Pei Wen Liao, 廖珮妏
• Other Authors: C. S. Lai
• Format: Others
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/94015063125535123325

碩士 === 長庚大學 === 電子工程學系 === 100 === Inflammasomes are wellknown as the key regulators of the innate immune response triggered by tissue damage or other microbial stimulator, and the activity of these multi-protein complexes has been linked to common autoinflammatory . Inflammasomes activate the proinflammatory cytokines interleukin IL-1β. In addition, potassium efflux has been linked to the activation of inflammasomes. In this research, a novel method was proposed to detect the inflammasome activation from extracellular potassium ion concentration. The sensor platform was designed as the electrolyte insulator semiconductor (EIS) structure with fluorinated HfO2 sensing membrane. The potassium (K+) sensitivity of HfO2-EIS structure is 2.23 mV/pK in the concentration between 10-5 M and 1 M. For the samples with fluorine (F19+) ion implantation, the pK sensitivity can be effectively improved to 75.58mV/pK. The variation of K+ concentration under treatment with the nigericin was 65.92 mV. It takes the advantage of directly monitoring the alteration of K+ as the measurement indicator of inflammasome activation.