| Summary: | 碩士 === 國立嘉義大學 === 園藝學系研究所 === 100 === Abstract
Background and Aim Because of the overexploitation of wild species and their habitat destruction, members of the genus Paphiopedilum are under potential threat of extinction. Micropropagation has been successfully employed for mass production of other orchids such as Phalaenopsis at an industrial level but there is no suitable protocol for Paphiopedilum. This study purpose in Paphiopedilum is to conduct a serials of preliminary but pioneering experiments to go toward that achievement of Phalaenopsis in micropropagation.
Methods To increase the quantity of available materials for in vitro culture, potted plants of a Maudiae-type hybrid and the species barbatum of Paphiopedilum were applied with different concentrations of 6-benzylaminopurine (BA) and gibberellic acid (GA3) for enhancement of lateral shoots emergence and stem elongation respectively. In micropropagation of a complex-type Paphiopedilum hybrid through lateral shoot culture, PPMTM (Plant Preservative Mixture) was used to sterilize and “cutting in half longitudinally” technique was used to increase propagation efficiency. Characteristics of in vitro shoot multiplication through Paphiopedilum inflorescence tips culture were also observed under microscope once every two weeks.
Key results
1. The application of BA on potted mother plants could enhance number of new shoots induced, from 2- 3 in control to 4- 5 in the treatments of BA.
2. The application of GA¬¬3 on potted plants caused stem elongation and there were about five stem node segments from each elongated shoot were used as explants for in vitro culture.
3. An effective method to sterilize lateral shoots was obtained by using PPM™.
4. “Cutting in half longitudinally” technique in lateral shoots represented an impressive number of shoots per explant after 7 months of culture with 18.78 shoots. In uncut treatment, there were only 2.67 shoots induced until then.
5. The in vitro culture of inflorescence tips shown different results among different species and hybrids. Survival percentage of explants at stage 2 (see details in content) was likely higher compared to other stages. Time for shoot formation varied from 2.3 to 8.3 months. Three types of shoot formation were observed, one shoot per explant, multiple shoots formation at location 1 and location 2 (see details in content).
Conclusions This study achieved some goals in micropropagation using lateral shoot explants of Paphiopedilum through increasing the quantity of available materials, sterilization and propagation efficiency. Shoot formation process in the in vitro culture of Paphiopedilum inflorescence tips also was recorded.
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