Early Identification Technique on Cell Apoptosis: AFM Application

• Main Authors: Chun-Hung Chen, 陳俊宏
• Other Authors: Dau-Chung Wang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/50926473726243397690

碩士 === 國立雲林科技大學 === 機械工程系碩士班 === 99 === In recent years, a thorough description of single cell’s responses and morphology is made possible due to advanced development on nano-characterization technique. AFM has been a tool adapted to this development since its high resolution and simple need in sample preparation. But AFM has its limit in the repositioning of the probe over the same cell, after moving away from it. By integrating a self-designed sample stage, a cell identification grid and image software technique, a cell finding mechanism was developed in this research. The identification of apoptotic cells were assisted by CCD’s image of a fluorescence microscope. As a conclusion, under the help of sample stage CCD, AFM could identify a single cell and acquire its membrane information. The cell apoptosis was induced by H2O2. This study correlated cell membrane roughness of Lymphocyte with its apoptotic status.