Study on Array Lactate Biosensor

• Main Authors: Hung-yu Wu, 吳竑褕
• Other Authors: Jung-chuan Chou
• Format: Others
• Language: en_US
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/95192180766020997078

碩士 === 國立雲林科技大學 === 光學電子工程研究所 === 99 === In this thesis, the pH-sensor based on a ruthenium dioxide (RuO2) electrode was investigated, and which enzyme immobilization with covalent bond method was used to prepare the array lactate biosensors. In this thesis, the device characteristics of the potentiometric lactate biosensors were investigated and improved. Moreover, the electrochemical impedance spectroscopy (EIS) was utilized to investigate the modification of electrode on the lactate biosensor. Besides, the lactate dehydrogenase (LDH), coenzyme (Nicotinamide Adenine Dinucleotide, NAD+) with or without mediator (Ferricyanide) were co-immobilized on the pH sensitive membrane by 3-glycidoxypropyltrime thoxysilane (GPTS). After the reactions of enzyme, the resulting product, H+, was detected by the RuO2 thin film. According to the experimental results, the sensitivity of the potentiometric lactate biosensor (with ferricyanide) based on the RuO2 electrode is about 53.96 mV/mM in the linear range between 0.2 mM and 4 mM of lactate concentration, as well as, the response time (t95%) of the lactate biosensor is about 40 seconds. On the other hand, the sensitivity of the lactate biosensor based (without ferricyanide)on the RuO2 electrode is about 51.27 mV/mM and response time (t95%) of the lactate biosensor based on the RuO2 electrode is about 0.2 seconds. In this thesis, the potentiometric lactate biosensors based on the RuO2 thin film has some advantages, such as easy fabrication process, disposable, low cost. Furthermore, the lactate biosensor is applicable for measuring the lactate concentration of drinks in the future.