| Summary: | 碩士 === 國立陽明大學 === 生醫光電工程研究所 === 99 === The utilization of stimulated emission signal offers a variety of advantages over conventional linear optical contrast in imaging, especially for the identification of biological specimens with high spatial resolution in three dimensions, such as STED. However, the coherent aspect of stimulated emission has yet to meet its potential.
In this work, we have implemented a novel imaging method for long-distance fluorescence detection that is based on stimulated emission. Traditional fluorescence imaging relies on high numerical aperture (NA) optics in collecting non-coherent fluorescence efficiently. For comparison, the stimulated emission process can effectively convert the fluorescence signal into a coherent one. In this way, long-distanced detection can be realized with low NA optics, which may lead to renewed and expanded applications of fluorescence.
We have demonstrated the detection of fluorescence at long distance through stimulated emission. A scanning unit and a projection lens are integrated for long-distanced imaging. An excitation beam and a stimulating beam are combined to enable stimulated emission, while a lock-in amplifier is used to discriminate the stimulated emission signal
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