Application of surface enhanced Raman scattering (SERS) to distinguish bacteria species and Ampicillin resistance of Escherichia coli in urinary tract infection

• Main Authors: Yi-Ting Chou, 周宜霆
• Other Authors: Huihua Kenny Chiang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/41917923987958039507

碩士 === 國立陽明大學 === 生醫光電工程研究所 === 99 === Surface-enhancement Raman scattering (SERS) spectroscopy is a very useful method for rapid detection and identification of bacteria by their specific fingerprint. This study is using surface enhancement Raman scattering spectroscopy by silver nano-particle. We develop rapid identification for UTI with simple sample preparation, high reproducibility, and high sensitivity. We build Ag-SERS database about bacteria usually seen in UTI, including E. coli, Klebiselleae spp., Enterobacter spp. and Staphylococcus aureus. We compared susceptibility test for Escherichia coli (E. coli) at the same time. Using the peak intensity of SERS spectrum at 1460 cm-1, we determine the Ampicillin susceptibility for E. coli.