The Physiological Roles of Connective Tissue Growth Factor in Human Primary Synoviocytes

• Main Authors: Gu, Fang-Ru, 古芳如
• Other Authors: ChangCheng-Chi
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/68210028813502144883

碩士 === 臺北巿立體育學院 === 體育與健康學系碩士班 === 99 === The Physiological Roles of Connective Tissue Growth Factor in Human Primary Synoviocytes Abstract Purpose: The physiological roles of connective tissue growth factor (CTGF) in human primary synoviocytes. Methods: Human synovial tissue was primary cultured, and treated with rCTGF (100 ng/mL). Here we investigated the function of CTGF in proliferation, migration, and differentiation on human synoviocyte. Bone morphogenetic protein family was examined by RT- PCR and Western blotting assay. To exam the signal transduction pathway, phosphorylation status of mitogen-activated protein kinase (MAPK) and PI3 kinase (PI3K) proteins would be checked after CTGF treatment with Western blots. Results: We found that CTGF had no significant time- or dose- dependent effects in cell proliferation and migration in human synoviocytes. However, rCTGF treatment showed ability to differentiate into osteogenic cells, but not chondrogenic or adipogenic cells after indicated times. To screen the BMP family, the data demonstrated that rCTGF increased BMP2 mRNA and protein expression. In signal transduction, phosphorylated MAPK/p38 markly increased after rCTGF treatment, but phosphorylated JNK was decreased. Conclusion: Our data demonstrated that CTGF had no effect in proliferation and migration of human synoviocytes, but could promote osteogenesis differentiation. We suggested that CTGF-induced osteogenesis differentiation possibility by MAPK/p38 signaling pathway. In perspective, CTGF is a potent molecular protein drug to induce osteogenesis in articular disease. Key words: CTGF, synoviacytes, differentiation, BMP2, osteogenesis