Summary: | 碩士 === 東海大學 === 畜產與生物科技學系 === 99 === Two trials were conducted to investigate the effects of different level or carbon number of medium-chain Triglycerides on growth performance, gut flora and fat deposition of broilers. Trial 1, 600 newly hatched broiler chickens were allotted to 4 treatments, 6 replicates per treatment and 25 chicks per replicate. Chicks were fed diets containing 8% soybean oil and MCT (containing 24.9, 47.6 and 26.8% of C6:0, C8:0 and C10:0, respectively) mixtures: (1) 100% soybean oil; (2) 80% soybean oil + 20% MCT; (3) 50% soybean oil + 50% MCT or (4) 20% soybean oil + 80% MCT. Growth performance, fatty acids and protein digestibility, abdominal fat cell size and number, and gut flora were measured. The results showed that the weight gain and feed intake of broilers during 1-18 days of age were lower than those of control group when MCT replaced 50 and 80% of soybean oil, respectively (P<0.05). Weight gain and feed intake of broilers during 0-36 days of age were lower than those of control group when MCT replaced 80% of soybean oil (P<0.01). Feed efficiency and mortality were not different among treatments. Abdominal fat/body weight was lower than those of other treatments when MCT replaced 80% of soybean oil (P<0.01). The digestibility of medium-chain triglycerides were higher than 77.46%. Increasing dietary MCT had a tendency to increase of the digestibility of protein (P=0.11). Abdominal fat cell size was smaller than those of control group when MCT replaced more than 20% of soybean oil (P<0.01). Cell number was not different among treatments. Crop Lactobacillus+E. Coli total counts were lower than those of other treatments when MCT replaced 80% of soybean oil (P<0.05). Caecum E. coli and Lactobacillus+E. Coli total counts were lower than those of control group when MCT replaced 20% of soybean oil (P<0.05). Trial 2, 300 newly hatched chicks were allotted 3 treatments, 4 replicates per treatment, and 25 chicks per replicate. Chicks were fed diets supplemented 4% of (1) soybean oil; (2) coconut oil (containing 48.5% C12:0) and (3) C6:0 oil (containing 40% C6:0). Abdominal fat cell size and number and gut flora were measured. The results showed that abdominal fat cell size and number were not different among treatments. Ileum Lactobacillus, E. Coli and Lactobacillus+E. Coli total counts were lower than those of control group when dietary supplementation of C6:0 oil (P<0.01), but coconut oil had no affect. In conclusion, dietary supplementation of 1.6% MCT decreases abdominal fat cell size and inhibits caecum E. Coli counts. Increasing dietary MCT to 6.4% decreasing feed intake, weight gain and abdominal fat deposition while do not affect the feed efficiency and mortality of broiler. Dietary supplementation of C6:0 oil inhibits ileum flora but do not affect abdominal fat cell size and number of broilers. Dietary supplementation of C12:0 neither affect gut flora nor abdominal fat cell size and number of broilers.
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