Studies on the analysis of glyphosate and human serum ferritin by CdSe quantum dots electrochemiluminescence biosensors

• Main Authors: Gu-Yu Kuo, 郭家瑜
• Other Authors: Cheng-Wen Whang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/05007806335166550847

碩士 === 東海大學 === 化學系 === 99 === This studies has discussed to modify CdSe quantum dots (QDs) on screen-printed carbon electrodes, and conjugate to antibody detected glyphosate and human serum ferritin, developing a QDs electrochemiluminescence (ECL) biosensor. First a carbon Nanotube (CNT) and chitosan (CHIT) mixture coating on electrode surface, forming a CNT-CHIT thin film , then the surface modified thioglycolic acid (TGA) CdSe/TGA QDs is fixed in the thin film. use (3-Aminopropyl)triethoxysilane (APS) and N-succinimidyl-4-(maleimido- -methyl) cyclohexanecarboxylate (SMCC) to link antibody with the QDs on electrode surface. When antigen in sample solution reacted with antibody producing immunocomplex , will cause changed of the QDs surface state , result in ECL intensity decayed. This dissertation has two parts: The first part detects Gly by the QDs cajugated Gly antibody modified screen printing carbon electrode, examines the mensurable range is 0.01~20.0 ng/mL, the linear correlation coefficient (R2) 0.9906. The second part replace the screen printing carbon electrode surface QDs conjugated antibody by ferritin antibody. Detecting human serum ferritin mensurable range is 0.1~20.0 ng/mL, the linear correlation coefficient (R2) 0.9952, detection limit 0.07 ng/mL. Observes in the blood 5 kind of possibly influential species, including: The human serum albumin, Alpha-Fetoprotein, human hemoglobin, human transferrin, ferric chloride. The influence of those species are not serious of our method. To analyze in 10 volunteer blood serum's ferritin result compared with the ELISA method, under 95% confidence degree, those result has good Similarity. Demonstrated that the QDs ECL biosensor sensing ferritin has the high sensitivity, high accuracy, simultaneously cost not expensive, and may produce once a lot.