Effect of medium additives on the formation of bioactive components in the submerged culture of Antrodia cinnamomea

• Main Authors: Chuang,Yatin, 莊雅婷
• Other Authors: Yang,Fanchiang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/72852373309715890968

碩士 === 東海大學 === 化學工程與材料工程學系 === 99 === Antrodia cinnamomea is an endemic medical mushroom in Taiwan. It is well known that the major effective components in this medical fungus are polysaccharides, steroids, flavonoids, phenols and triterpenoids. The bioactive and efficacy of A. cinnamomea are still the main focus in many researches. In this research different kinds of citrus peel’s water extracts were added into the media to investigate their effects on the formation of bioactive components of A. cinnamomea in shake-flask cultures. The most effective kind and the optimal concentration of citrus peel’s water extracts were determined. Moreover, addition timing was also studied. The results showed that lemon peel’s water extract could effectively increase bioactive components. The intracellular polysaccharide value reached to 230.77 mg/g D.W. at 8 day. The crude triterpenoid and total crude triterpenoid were 20.42 mg/g D.W. and 28.29 mg/100 ml, respectively at the 20th day. The flavonoid value was 3.22 mg/g D.W. at the 28th day. Concerning the effects of different concentrations of lemon peel’s water extract, the optimal concentration was determined to be around 10ml. The crude triterpenoid and total crude triterpenoid could reach to 29.53 mg/g D.W and 41.79 mg/100 ml respectively at the 28th day. About addition timing, the day 12 for the addition of 10 ml lemon peel’s water extract were recommended and the flavonoid and total phonel rose to 4.63 mg/g D.W. and 22.01 mg/g D.W., respectively at the 28th day. The results of this study are expected to be used for the enhancement of bioactive components production in the submerged culture of A. cinnamomea. Key words: Antrodia cinnamomea, intracellular polysaccharide, crude triterpenoid, flavonoid.