Isolation and Functional characterization of three putative Lysozyme Genes in Acinetobacter baumannii

• Main Authors: You-Jie Chen, 陳宥傑
• Other Authors: Kai-Chih Chang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/06261783398990269743

碩士 === 慈濟大學 === 醫學生物技術研究所 === 99 === The phage lytic enzymes (or lysins) have received a great deal of attention as novel anti-infective agents. The number of known genes encoding these peptidoglycan hydrolases has increased markedly in recent years, due in large part to advances in DNA sequencing technology. As the genomes of more and more bacterial species/strains are sequenced, lysin-encoding open reading frames (ORFs) can be readily identified in lysogenized prophage regions. In the current study, we sought to find lysozyme genes for Acinetobacter baumannii. The sequenced genomes of Acinetobacter baumannii ATCC17978 strains were mined for lysozyme ORFs. Of these lysozymes, three putative lysozyme genes were chosen for recombinant analysis. Following expression and purification, various biochemical properties of A1S_0187, A1S_1600, A1S_2016 were determined in vitro, including anti-bacteria ability and enzyme activity. The enzymes exhibited activity at low concentrations. These lysozymes were active against multidrug-resistant Acinetobacter baumannii tested, with virtually no activity against other nonacinetobacter species. Under the Scanning Electron Microscope, they were observed that these lysozymes can directly destroy A. baumannii. A1S_1600 and A1S_2016 shows potential for development as an enzybiotic agent.