Analysis of the interaction between P-bodies and stress granules under heat stress

• Main Authors: Szu-Jing Huang, 黃思淨
• Other Authors: Ming-Der Lin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/65349966070420193786

碩士 === 慈濟大學 === 生命科學系碩士班 === 99 === Regulation of mRNA translatability and stability are critical for the cell survival in stress conditions. In mammalian cells, stress granules and P-bodies are distinct RNP granules with different functions. Stress granules are responsible for mRNA storage in stress conditions while P-bodies are for 5’ to 3’ mRNA degradation. In Drosophila, it has not been proved whether P-bodies and stress granules possess distinct function or not. Our previous results indicate that dDcp1, a P-body component, aggregates around nurse cell’s nuclei in heat stressed egg chamber. We hypothesize that Drosophila P-bodies may possess mRNA storage function as mammalian stress granules in heat stress. To address whether heat stress can induce the co-aggregation of Drosophila P-body components with stress granules in nurse cells, we constructed GFP-tagged Drosophila homologs of mammalian canonical stress granule components including PABP, Rasputin, Trip1 and Rox8. Similar to mammalian stress granule, GFP-tagged stress granule components are able to congregate in nurse cell cytoplasm in response to heat stress. Our data also indicate that Drosophila stress granule components could colocalize with dDcp1 and Pacman. These results imply that dDcp1 containing P-bodies change its contents and coaggreate with stress granules in response to heat stress.