Establishing the Quantitative Evaluation System of hindlimb function in the SD Rats of Spinal Cord Injury based on Image Technology

• Main Authors: Jan,Min-Ji, 詹閔機
• Other Authors: Chen,Pei-Jarn
• Format: Others
• Language: zh-TW
• Published: 100
• Online Access: http://ndltd.ncl.edu.tw/handle/90132354738902174536

碩士 === 南台科技大學 === 電機工程系 === 99 === According to the published literatures showed that the evaluation of animal experiments currently determined by different techniques such as primary open-field test, BBB test, open-field activity test, automatic walkway test, footprint analysis test…etc. However, most of the methods mentioned above are high dependent on the subjective observation during to observe and track. In this study, the SD (Sprague-Dawley) rats with spinal cord injury will be marked four joints of hindlimb and dual cameras were used for top angle and side angle snapping. The images captured by top camera were used to show the tracks of SD rat locomotion after image processing, and then to analyze the walking speed of rats. Furthermore, the images captured by side angle camera were used to track the four joints’ movement and to calculated the angles between the joints to determine the BBB score of SD rats with spinal cord injury and determined the status of recovery. In addition, the relationships between the quantitative parameters and the BBB score were indicated, and the errors caused by different shooting angle were also discussed. Finally, the system based on image technology developed in this study could be improved to others animal experiments.