Effects of Etancercept in the central nervous system of traumatic brain injury rat model

• Main Authors: Chong-Un, Cheong, 張仲元
• Other Authors: Ching-Ping, Chang
• Format: Others
• Language: zh-TW
• Published: 100
• Online Access: http://ndltd.ncl.edu.tw/handle/04407103745810809275

碩士 === 南台科技大學 === 生物科技系 === 99 === After traumatic brain injury(TBI), nerve tissue will continued from hours to weeks under primary and second insult of neural damage. Glial cells respond to all forms of central nervous system insults such as infection, trauma, ischemia and neurodegenerative disease by a process commonly referred to as reactive astrogliosis, which involves changes in their molecular expression and morphology, and in severe cases, scar formation. Pro-inflammatory cytokines, such as: TNF-α, IL-1-β and IL-6 will increase within few hours after the injury. Cytokines can either promote this neurotoxicity, by encouraging excitotoxicity and propagating the inflammatory response, or attenuate the damage through neuroprotective and neurotrophic mechanisms, including the induction of cell growth factors. Then recruited white blood cells and platelets in small blood vessels, induced blood brain barrier damage and cerebral edema leading to reduced brain perfusion that related to secondary brain damage. This study utilized the rat model of fluid percussion injury model to study TNF-α in the core, penumbra, hippocampus, paraventricular and hypothalamus region before and after Etanercept treatment. The aim of this study was to analyze Etanercept could have neuroprotective effect of TBI rat induced by fluid percussion injury (FPI). Animals were separated by three groups: (1)sham operation group, (2)TBI control group saline (1ml/ kg per 12 hours for 3 days, via IP inj. after FPI formation) and (3)TBI combined with Etanercept treatment group ( Etanercept5mg/kg per 12 hours for 3 days, via IP inj. after FPI ). This study discusses the use of Etanercept (TNF-α antagonist) ability to protect the central nervous system cells and the use of immunohistochemistry to identify Etanercept in the role of cytokines . Another focus of this article with BrdU and doublecortin system to observe the neurogenesis, microglial cells and astrocyte fusion was measured cytokine TNF-α effect. Observed DCX (doublecortin), NGF (neuron growth factor), VEGF (vascular endothelial growth factor) to and their changes in brain nerve cells. Etanercept can produce neuroprotective and neurogenesis and may offer another new page on nerve regeneration research.