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• Main Authors: Cheng-Yi Wang, 王晸怡
• Other Authors: Bea-Ven Chang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/30837449706686255736

碩士 === 東吳大學 === 微生物學系 === 99 === 　　Sewage treatment process, organic waste contain toxic chemicals, such as nonylphenol(NP), bisphenol A(BPA), bisphenol F(BPF), tetrabromobisphenol A(TBBPA), polybrominated diphenyl ethers(PBDEs) and tetracycline antibiotics(TCs), accumulated in the sewage treatment sludge.Many studies have shown that adding the ability of strains and the degradation can remove organic toxic chemicals. However, degradation of toxic chemicals with the ability to add the strain, the effects of environmental condition may did not to play its ability to degrading. 　　In this study, micro-entrapment technique to observe the degradation of lignin enzymes and the ability of the strains with embedded or microentrapment through a variety of organic toxic chemicals on the ability to remove the effect. Strains used in this study, is isolated from ours laboratory in the past with good of nonylphenol removal capacity of the strains: Arthrobacter nicotianae (CT1), Bacillus thuringiensis (CT2 ), Bacillus sphaericus (CT7) and adapted one years mixed flora. And some isolation of bisphenol A and bisphenol F with good removal capacity of the strains, Bacillus sp. (L2) and isolated from sediments of the strain has a good ability to remove dibromo-diphenyl ether, Pseudomonas sp. (W4). 　　The results showed in this study that different sources of extracellular ligninolytic enzyme form solid-state culture or spent mushroom compost (SMC) substrate. NP has the ability to degrade will be directly added to the sludge of the strains in their ability to remove NP of CT7 and CT2> CT1, but after the removal of entrapment strain CT7> CT2, there are clearly a good play of its NP removal. It is entrapment in the way that you can use to enhance the ability of the strains with NP decomposition ability to adapt to the environment and competitiveness.Added directly to different sources of extracellular ligninolytic enzyme on the removal of NP, solid-state culture is greater than SMC; But after calcium alginate entrapment, its the removal of NP, SMC > solid-state culture . 　　The removal of different toxic chemicals in the ability of NP with the degradation of strains, can also play against other toxic chemicals removal effect, but still containing bromine use of toxic chemicals with the ability of the strain degradation better bromide . Comparison micro-entrapment, entrapped and non-entrapped three treatments found that strains of micro-entrapped removal efficiency greater than entrapped and non-entrapped , and entrapped of micro- entrapment removal non- entrapped much larger than the entrapped. 　　The use of enzymes, micro-entrappedcapsule is able to effectively remove a variety of organic removal efficiency of toxic chemicals with the toxic chemicals but vary the structure, ligninolytic enzyme removal efficiency of different toxic chemicals of the comparison, BPF> BPA> NP>CTC> OTC = TC> TBBPA> BDE209> BDE15. 　　The study found that the use of micro-entrapped technology, microentrapp- ed ligninolytic enzyme does not promote its removal efficiency, but can be exte- nded to remove organic toxic chemicals of time, although it can not achieve the purpose of re-use, but may be extended processing time. Micro-entrapped techn- ology to the bacteria with the ability to degrade will increase the resistance on the environment and competitiveness, and thus the ability to play the good of the removal efficiency. 　　Therefore, the use of microbial degradation of organic toxic chemicals, the microentrapped bacteria microcapsules can be used to increase the removal effi- ciency. And added ligninolytic enzyme degradation and the ability of a strain, a combination of both advantages and disadvantages. 　　To sum up, in the treatment of organic toxic chemicals, the ligninolytic enz- yme and decomposition ability of the bacteria with microcapsules to increase the removal efficiency.