| Summary: | 碩士 === 東吳大學 === 微生物學系 === 99 === Whitr-rot fungi can degrade lignin. There are three enzymes have the ability to degrade lignin: laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP). Removal of environmental pollutant was investigated with several white rot fungi. This research in view of the Pleurotus eryngii comparison with the enzyme which produced in the solid state and liquid state culture to biodegradation the environment common pollutant nonylphenol(NP), Bisphenol A(BPA), Bisphenol F(BPF), tetrabromobisphenol A(TBBPA), dibrominated diphenyl ether(DBDE). The result showed that the P. eryngii may efficient removal pollutant under two cultivation ways.
Under the liquid state culture with P. eryngii strain BCRC P1 and P. eryngiistrain BCRC 36163 during 18 days of culture, laccase acivity can reach 223.9 U/L and 138.7 U/L, respectively. The laccase acivity can reach 166.3 U/L of P. eryngii strain BCRC 36213 during 20 days of culture. P. eryngii strain BCCRC 36213 liquid state culture was completely removed 20 mg/L NP within fourteen days inculation. By adding MnSO4 (0.5 mM), CuSO4 (1 mM), guaiacol (1 mM), VA (1 mM), gallic acid (1 mM), H2O2 (5 mM), tartaric acid (20 mM) and citric acid (20 mM), during 14 days of culture, the relative remove rate of 20 mg/L NP tartaric acid (20 mM) and citric acid (20 mM) was inhibited remove. The fastest remove of adding MnSO4 (0.5 mM) of 20 mg/L NP can reach 94.9 % within eight days inculation. The others of remove of 20 mg/L NP by adding CuSO4 (89.5%), guaiacol (87.8 %), VA (86.9 %), gallic acid (91.7 %), H2O2 (86.4 %), tartaric acid (61.7 %) and citric acid (64.8 %). Extraction rude enzyme by culture fluid add ABTS (1 mM), during three hours of culture, remove of 20 mg/L NP can reach 96% under pH 6 and 30℃.
Under the solid-state conditions with P. eryngii strain BCRC 36213 during 30 days of culture, laccase acivity can reach 39.3 U/g. Extraction rude enzyme by solid-state culture add ABTS (1 mM), during three hours of culture, remove of 20 mg/L NP can reach 100% under pH 6 and 30℃. During solid state culture, the laccase activities in the different matrixs is sawdust and wheat atraw add 1mM MnSO4 (25.4 U/g), sawdust and wheat atraw (17.3 U/g), sawdust and rice bran (13 U/g) and manganese peroxidase is sawdust and wheat atraw add 1mM MnSO4 (23.8 U/g), sawdust and wheat atraw (14.9 U/g), sawdust and rice bran (12.1 U/g) respectively. Extraction rude enzyme by solid-state culture via purification of protein enzymes (salting out and dialysis) that laccase and MnP activity can reach 68.4 U/g and 103.3 U/g, remove of 20 mg/L NP can reach 39.1% within one hour inculation. It add ABTS(1 mM) during one hour culture remove of 20 mg/L NP can reach 88.1%. Extraction rude enzyme by solid-state culture via purification of protein enzymes (salting out and dialysis), during one hour culture, remove of 2 mg/L in the different oganic compost is BPA(97.3%), BPF(85.7%), TBBPA(59%), remove of 20 mg/L in the different oganic compost is BPA(98.4%), BPF(93.1%), TBBPA(86.2%). Those oganic composts can completely remove within twenty-four hours inculation. But DBDE can`t remove.The sum up, P. eryngii strain BCRC 36213 under the solid state culture by adding MnSO4 (0.5 mM)can promote produce an enzyme activity. When P. eryngii strain BCRC 36213 add ABTS(1 mM) can increase rate of remove oganic composts. Extraction rude enzyme by solid-state culture via purification of protein enzymes (salting out and dialysis) can increase enzymes product and remove efficiency. This research offers the feasible methods for removal of oganic composts for bioremediation.
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