Screening and identification of Chlorella sorokiniana fractions of high activities in inhibiting proliferation of human colon adenocarcinoma cells and modulating TGF-β1 secretion of human foreskin fibroblast

• Main Authors: Yu-Ting Huang, 黃鈺婷
• Other Authors: Phoency Lai
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/29179810603438543570

碩士 === 靜宜大學 === 食品營養研究所 === 99 === Among Chlorella extracts, hot-water soluble extract accounts for 16-18%, the polysaccharide or glycoprotein from cellulose hydrolysate accounts for 8%, leaving the insoluble part of ~60%. This study was to isolate hot-water soluble extracts (WS) and insoluble (WIS) components of Chlorella sorokiniana with various extraction conditions, including enzymatic treatments (cellulase, chitinase and tannase), different solvents (ethyl acetate and n-hexane), and fractionation (WS > 10, 1-10 and <1 kDa). The chemical compositions (monosaccharides, phenolics, and organic acids) the inhibition effect on human colon adenocarcinoma cell (Caco-2) and stimulation on TGF-β1 secretion of human foreskin fibreskin fibroblast (Hs68) were examined on the extracts and selective fractions. The results of monosaccharide composition analysis indicated that the sugars in WS and enzyme hydrolysis fraction are mainly glucose and galactose, following by ribose and mannose. The results of polyphenolic compounds showed that the direct solvent extracts (EaS and HS) and the solvent extracts of WIS (WIS-EaS and WIS-HS) exhibited an order that lutein, HS > WIS-HS > EaS > WIS-EaS. The extraction efficiency (based on total polyphenolics yield) of n-hexane was greater than that of ethyl acetate. The tannase hydrolysate of WIS (WIS-TaS) had an significantly antitumor activity against Caco-2 cells (at 0.1 mg/mL, inhibition: 57.23% ). Mixture of TaS and gallic acid exhibited a high inhibitory effect (78.4% at 0.2 mg/mL). The WIS-EaS stimulated Hs68 proliferation (134% at 5 μg/mL) and TGF-β1 secretion, more significantly than did the WIS-HS. In conclusion, WIS-TaS exhibited an excellent inhibition activity on Caco-2 cells. Gallic acid exhibited the best cell inhibition activity against Caco-2 among the polyphenolics examined. The WIS-EaS and WIS-HS stimulated TGF-β1 secretion from Hs68, implying the potential in wound healing.