Effect of Lycium barbarum polysaccharides on antioxidative status and anti-aging in senescence accelerated mice

• Main Authors: Chia-lung Chao, 趙佳容
• Other Authors: Ming-fu Wang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/63295630765961972709

碩士 === 靜宜大學 === 食品營養研究所 === 99 === Lycium barbarum L. is a traditional Chinese herbalmedicine. Previous studies have demonstrated that Lycium barbarum polysaccharides (LBP) show antioxidant, antihypertensive and antitumor effects. The purpose of this study was to investigate the effects of Lycium barbarum polysaccharides on learning and memory ability, and antioxidant status in senescence-accelerated mice (SAMP8). 3 and 6-month-old SAMP8 male and female mice were used in this study. SAMP8 mice were randomly divided into five groups, where each group contained 10 mice, including control group, 0.42 ml LBP group, 0.84 ml LBP group, 1.67 ml LBP group, and 4.20 ml LBP group. This study period lasted for 12 weeks. Besides food intake, the body weight was also measured. The 11th week, the open field activity test was recorded in evaluation for behavior, and the aging scores were measured. One week before scarification, the single-trail passive avoidance test and active shuttle avoidance test were performed. The biochemical parameters of serum were analyzed after the mice sacrificed. The antioxidative enzyme activities were estimated in the liver, and the β-amyloid of brain was observed. The results showed that there was no significant difference in the body weight, food intake, and locomotion among each group. The aging score of control groups was significantly higher than experimental groups in 3 and 6-month old mice (p<0.05). Results from ingle-trail passive avoidance test and active shuttle avoidance test showed that experimental groups had significantly better learning memory ability (p<0.05). Activities of antioxidant ability, including SOD, GPx, catalase, and total thiol, in the experimental groups were significantly higher than that of control groups (p<0.05). The MDA concentration and protein carbonyl in the experimental groups were significantly lower than the control groups (p<0.05). Besides, the β-amyloid protein deposition of brain were significantly lower in the experimental groups than control groups (p<0.05). In summary, the supplement of Lycium barbarum polysaccharides may promote the antioxidant defense system, reduce oxidative stress-induced damage, reduce the accumulation of β-amyloid deposition, and improve learning and memory ability.