| Summary: | 碩士 === 靜宜大學 === 食品營養研究所 === 99 === Tinnitus is a phantom sensation of sound in the absence of external stimulations. Serious tinnitus may affect emotion, concentration, and even the quality of life. However, the treatments of tinnitus need further study. The purpose of this study was to develop a behavioral model of conscious tinnitus with SAMP8 mice induced by salicylate, and evaluated the effects of Spiulina water extract on relative receptors. Three-month-old SAMP8 mice were randomly divided into normal control, tinnitus with intraperitoneal injection of salicylate (300 mg/kg BW), and Spiulina water extract (1000 mg/kg BW) with salicylate-induced tinnitus groups. Body weight were recorded and active avoidance test were performed during the experimental period. The biochemical values was analyzed after sacrificed, and the cannabinoid receptor 1 (CB1 receptor), vanilloid receptor type 1 (VR1 receptor), dopamine D1A receptor and γ-amino butyric acid (GABA) Aβ3 receptor mRNA expressions of brain stem, frontal lobe, hippocampus, temporal lobe and inner ear were also examined. Our results demonstrated that both salicylate and Spiulina water extract did not affect the liver and kidney functions of SAMP8 mice. Salicylate-induced tinnitus led significant increase in the number of false positive response while the percentage of correct response tented to reduce and Spiulina water extract could improve the trend. Salicylate significantly reduced the cannabinoid receptor 1 (CB1 receptor) and γ-amino butyric acid (GABA) Aβ3 receptor mRNA expression in brain stem, frontal lobe, hippocampus, temporal lobe and inner ear, while Spiulina water extract significantly increased their expression. In addition, salicylate significantly increased the dopamine D1A receptor expression in brain stem, hippocampus, temporal lobe and inner ear, and Spiulina water extract could significantly lessen their expression. Salicylate significantly increased the VR1 receptor expression in brain stem, frontal lobe, hippocampus and temporal lobe, and Spiulina water extract also significantly reduced the expression. In summary, we conclude that the Spiulina water extract could improve the salicylate-induced conscious tinnitus in SAMP8 mice, which might due to regulate the expression of tinnitus related receptor.
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