Application of Yeast Contain Rumen Microbial Bacteriocin on Feed Additive for Broiler and Laying Hen

• Main Authors: Wei-Yuan Shih, 史崴元
• Other Authors: Shu-Yin Wang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/95115547416470691714

碩士 === 中國文化大學 === 生物科技研究所 === 99 === The purpose of this study was to evaluate the effect of yeast with bacteriocin from Ruminococcus albus 7 on physiological state and production performance of broilers and hens. The broilers experiment were divided into treatments including (1) control, (2) yeast with bacteriocin (0.25%)[B], and (3) nosiheptide (2.5 ppm)[N]. The in vitro fermentation experiment was tested at the 3 and 5 week old by inoculating fecal and cecal content collected from control group. The gas production and fermentation product concentration were measured after 24 hr incubation. In broiler feeding experiments, clinic chemistry parameters and fecal flora test were assay at the third and fifth week. Broilers were slaughtered at the same age to get ceca content samples for volatility fatty acid composition and ammonia concentration assay. Both [B] and [N] treatments resulted in higher propionicacid concentration in fermentation broth. The gas production models showed high correlation between fecal and cecal inoculating samples, but no significant differences among all treatments. The clinic chemistry parameters indicated that addition of yeast with bacteriocin increased the blood uric acid at the 5 weeks old, but it decreased cholesterol concentration (about 18%)(P<0.05). The fecal flora results indicated that addition of yeast with bacteriocin decreased Salmonella(P<0.05) counts at 3 weeks old, the Salmonell and Coliform counts also decreased at 5 weeks old(P<0.05). However, the Lactobacillus counts were increased when yeast with bacteriocin added in diet. According to the data from clinic chemistry, fecal flora and fermentation performance, it showed that antibiotic and bacteriocin had similar effect on cecal fermentation product. Nevertheless, bacteriocins addition could reduce blood cholesterol level of broilers. In the laying hen experiment, animals were assigned into five groups including (1) control group, (2) yeast control (0.25%), (3) yeast with bacteriocin (0.125%), (4) yeast with bacteriocin (0.25%) and (5) yeast with bacteriocin (0.5%). All supplements were added to the experimental diets of the hens from 26 to 46 weeks old. Samples were collected every four weeks. Blood samples were collected from the wing vein for clinic chemistry assay and fecal samples were collected by swab for the flora test. The egg production performance was recorded daily and fresh eggs were collected for quality test. The clinic chemistry result indicated that the addition of yeast with bacteriocin decreased the AST (aspartate-aminotransferase) activity and cholesterol concentration in laying hen blood. The result of egg quality indicated that bacteriocin increased the mass of yolk and the strength of eggshell, but it had no effect on the performance of egg production. Low concentration (0.125%) of bacteriocin addition could decrease total yolk cholesterol, addition of 0.25% bacteriocinin diet could decrease the proportion of C16: 0andC18: 0 in egg yolk fatty acid composition. The fecal flora result indicated that the addition of bacteriocin increased the Lactobacillus counts. After supplement bacteriocin for 8 to 12 weeks, the Salmonella and Escherichia coli counts were also decreased. Combined the data from clinic chemistry, fecal flora, egg production and egg quality, the 0.25% yeast with bacteriocin addition may result in the best physiological parameter and egg production performance of laying hen.