Synthesis of Amylase-imprinted poly(ethylene-co-vinyl alcohol) magnetic composited nanoparticles for the applications of hydrolysis

• Main Authors: Yung-Chao Chen, 陳詠超
• Other Authors: Hung-Yin Lin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/06770536698432220409

碩士 === 國立高雄大學 === 化學工程及材料工程學系碩士班 === 99 === Molecularly imprinted polymers (MIPs) have frequently been employed as recognition elements in sensing applications, or for the controlled delivery of small molecule drugs. An equally important but less well stuied application is the use of MIPs in the binding and immobilization of active enzymes. In this study, magnetic MIPs (MMIPs) recognizing the enzyme amylase were prepared using phase inversion of poly(ethylene-co-vinyl alcohol) (EVAL) solutions with various ethylene mole ratios in the presence of amylase. The size distribution,specific surface area, magnetization, and composition were characterized by dynamic light scattering, Brunauer-Emmett-Teller (BET) analysis, superconducting quantum interference devices, and X-ray diffraction, respectively. The activities of both bound template and rebound enzyme was established by measuring glucose production via starch hydrolysis,at different temperature, for MIPs with different compositions (weight % of EVALs and mole % of ethylene ).Finally, the reusability of magnetic amylase-imprinted EVAL nanoparticles was examined and starch hydrolysis for 60 times at 60oC.