Multi-function Automatic Tissue Microarrayer for Pathological Analysis and Staining Reagent Concentration Tests

• Main Authors: Ting-Hsuan Chen, 陳庭軒
• Other Authors: Fang-Jung Shiou
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/jj452r

博士 === 國立臺灣科技大學 === 機械工程系 === 99 === This study aims to develop a multi-function automatic tissue microarrayer that incorporates functions accommodating both the pathological analysis and the staining reagent concentration tests. Furthermore, tissue alignment accuracy, tissue punching position and quantity planning, and tissue microarray block (TMA) package quality are also discussed. This study contains four stages. First, through evaluating functional requirements including both pathological analysis and staining concentration tests, the possible structure to design the apparatus is considered based on these concepts. In addition, a concept of automatic flow is also discussed in order to find out the necessary condition for achieving multi-function automation. A genetic algorithm (GA) is then performed to obtain the optimal translation and rotation parameters for image alignment to improve alignment accuracy. Next, a punching position and quantity planning is discussed using force-directed placement algorithm to continually modify the spring constant between components and establish the appropriate punching position and quantity. Finally, to improve the TMA block package quality, Taguchi method is employed for TMA process to obtain the optimal assembly process parameter. Experimental results revealed that self-developed tissue microarrayer can not only efficiently enhance tissue alignment accuracy but also completely plan tissue punching position and quantity. As for TMA process, the robust parameters can obviously improve the package quality of TMA block.