| Summary: | 碩士 === 國立臺灣大學 === 醫學工程學研究所 === 99 === The CD-based biochip has been developed for several years, not only for its low cost, mass production and ability to provide the centrifugal force to drive micro-fluid; but also for its light weight and friendly usage which allows normal CD-ROM to read the signals. All of those advantages have made it more competitive among general bio-optical chips. In this research, we use dip pen nanolithography (DPN) to produce nanoarrays paralleled on the CD’s storage track, and take the advantages of its accuracy and precision to make the nanoarrays’ array consistent with the CD track period, so that to make the detection of molecules on nanoarray approaching the memory capacity of the disc, and look forward to expand the detection of molecular species. In order to reach a lower limit of detection, we also deposit gold thin film on one dimensional nanostructure of CD’s storage track and immobilize gold nanoparticles. We discuss the phenomenon of localized surface plasmon resonance (LSPR) under this framework.
In order to develop molecular interaction part of this novel of nanoarray CD-based biochip, we first recognized immobilization of biomolecules process on the ultra-flat gold. The 250 nm latex beads conjugated with streptavidin has successfully immobilized on the gold film, and verified by atomic force microscopy (AFM). The results show that this method has potential to be used in the process of capturing viral particles. Then, we also deposited 50 nm gold thin film on the disk’s storage track and immobilized 60 nm gold nanoparticles, and used absorption spectra to discuss the LSPR effect. We found the LSPR performance of Blu-ray discs is the most significant one by comparing the characteristics of absorption spectra. Gold nanoparticles mixed with hydrogel. Finally, we use twelve DPN probe arrays to make hydrogel nanoarray on the disc. We acquire image and absorption spectra of the hydrogel on the storage array by dark field microscope and spectroscope. The absorption spectra show the effect of LSPR provide the possibility to enhance the signal of detecting the low concentrations of biomolecules.
In this study, we used parallel DPN process in protein arrays and applied the advantage of LSPR to amplify the signal in this new type of CD-based chip for molecular interactions. The microfluidic technology and CD-ROM reading methods will be developed toward the practical application in the future.
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