Plug Production of Brainea insignis (Hook.) J.Sm.

• Main Authors: Bi-Shuang Gao, 高碧霜
• Other Authors: Wen-Ju Yang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/00241218612644777064

碩士 === 國立臺灣大學 === 園藝學研究所 === 99 === 　　In Taiwan, there are more than 600 species of pteridophyte and some of them are native species; however, the evaluation of their potential in horticulture was very limited. Among the native species, Brainea insignis (Hook.) J.Sm. of Blechnaceae is of horticultural potential. The pteridophyte is often higher than 1 m and characterized with an erect stem and clustered single pinnate compound leaves. Moreover, the reddish new fond enriched its ornamental value. 　　Among the 4 sowing densities, 7.5, 15, 22.5, 30 spores/cm2, sporophyte formation and plug uniformity of Brainea insignis was affected by spore sowing density. The spore germination rates, 61%-76%, were not significant different among the sowing densities. The ratio of null plug (plug with no gametophyte) was less than 1% when the sowing density was higher than 15 spores/cm2. Besides, the sporophyte density was 0.6, 2.5, 7.9, and 7.5 sporophytes/cm2, respectively. Sowing density also affected gametophyte gender expression; the gender tended to develop into female under lower density and to develop into male under higher density. The ratio of female gametophyte reached 64% under 3 spores/cm2, and the ratio of male gametophyte tended to be 61% under 55 spores/cm2. In consideration of the sporophyte biomass, 15 spores/cm2 was suggested for the highest growth, 3 leaves per sporophyte and the leaf length was 1.78cm in average. 　　Nutrient requirement for sporophyte development of Brainea insignis was also investigated in this study. The ratios of plugs with no sporophyte were 59.9%, 56.6%, 29.3% and 98.2% for sowing and growing under 0%, 25%, 50%, and 100% Johnsons’ solution, respectively. Sporophyte/ plug, number of leaves/plug, fresh weight/ plug were highest when 50% Johnsons’ solution was supplied. Besides, the leaves under such nutrient condition were largest and with highest SPAD value. 　　Irradiance was acquired for spore germination of B.insignis. The germination rate was 2.5% under dark, but reached 72%-83% under 20, 31, 38 and 47 μmol•m-2•s-1 PPF. Male gametophyte rate decreased as light intensity increased. Under 47 μmol•m-2•s-1 PPF, sporophytes appeared earliest and the number of sporophytes per plug were the highest (6.4 sporophytes/plug). Besides, the sporophyte uniformity reached 93%. Two weeks of acclimation under 180 μmol•m-2•s-1 PPF was suggested for 3-leaves sporophyte before transplanting. The growth of young sporophyte (with 6-10 fronds) was much vigorous under 60 μmol•m-2•s-1 than under 90, 120, 150 and 200 μmol•m-2•s-1.