| Summary: | 碩士 === 國立臺灣大學 === 植物科學研究所 === 99 === Plants respond and adapt to stresses under stress conditions at the molecular and cellular levels. Transcriptional regulation of gene expression plays a fundamental role in response to environmental stimuli. A conserved sequence motif L/FDLNL/F(x) P was identified from the ERF proteins and SUPERMAN proteins that act as transcription repressors in Arabidopsis. This motif was named as the ERF-associated amphiphilic repression (EAR) motif, which was found to be essential for gene repression. In this study, the function of the SUPERMAN protein ZFP11 was investigated. It was found that ZFP11 was localized in the nucleus. ZFP11 can interact with HDA6 and HDA19 directly and the N-terminal of ZFP11 containing the zinc finger motif was responsible for the interaction. The zfp11 mutant carries a point mutation that changes one amino acid on the zinc finger motif of ZFP11. The zfp11 mutant plants displayed a dwarf and early flowering phenotype, suggesting that the zinc finger motif is important for the function of ZFP11. Compared with the wild-type, the zfp11 mutant plants show hypersensitive to ABA and NaCl in seed germination. Furthermore, the expression of ABI1 and ABI2 was decreased in the zfp11 mutant plants. These data suggested that ZFP11 may play an important role in plant ABA and salt responsive pathways.
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