| Summary: | 碩士 === 國立臺灣大學 === 植物科學研究所 === 99 === Pectin modification is catalyzed by a large enzyme family of pectin methylesterase (PME) residing in the cell wall. PME might modify the structure of pectin in order to adjust the characteristics of cell wall by pectin demethylesterification. In our previous study, we found that the activity of PME is induced by heat shock (HS), we further established the role of PME by retained plasma membrane integrity and coordinated with heat shock response (HSP) to confer acquired thermotolerance in planta. At least potential 66 members of PME genes are annotated in the Arabidopsis genome by a phylogenetic analysis. We tested 53 AtPME T-DNA insertion lines by thermotolerance assays, two mutants allele of AtPME34 (At3g49220) were characterized and revealed a consistent reduction in the acquired thermotolerance. The plasma membrane-localized of AtPME34 was transiently expressed in Arabidopsis protoplasts and onion cells. However, AtPME34 lacking the transmembrane domain showed the punctuated shape structures in the cytoplasm. We confirm again that the action of PME and PG during HSR might have a pronounced effect on the development of acquired thermotolerance. Furthermore, we demonstrated that the transcript of AtPME34 is probably regulated by HS–related genes such as AtHsa32 and AtHSFA3 involved in the HS response (HSR). In this study, we provide a genetic evidence, address that the unique AtPME34 plays a role during HSR to confer the acquisition of thermotolerance.
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