Molecular phylogenetic analysis of Alteromonadales species

• Main Authors: Cheng-Yu Hsu, 徐正育
• Other Authors: 謝文陽
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/92156629002236089197

碩士 === 國立臺灣大學 === 海洋研究所 === 99 === The 16S rRNA gene sequences of 245 type strains of Alteromonadales species and those of 22 strains belonging to unclassified group in the class γ-Proteobacteria have been collected in this study. To establish the effective phylogenetic relationship of these Alteromonadales strains, phylogenetic and signature nucleotide analyses, together with physiological and biochemical characterizations were conducted. Three algorithms, neighbor-joining method, maximum likelihood method and maximum parsimony method, were used to construct phylogenetic trees; the first two algorithms were incorporated in two models, Jukes-Cantor (JC) and Kimura Two-Parameter (K2P). All phylogenetic trees formed a stable Alteromonadales cluster that was divided into seventeen subclusters . The results indicated that eight new families might be established in the Alteromonadales. Moreover, Glaciecola lipolytica and Aestuariibacter litoralis could be reclassified as two different species in a novel genus . Our results also suggest that Pseudidiomarina is better retrieved and separated from the genus Idiomarina of the family Idiomarinaceae .