| Summary: | 碩士 === 國立臺灣大學 === 生醫電子與資訊學研究所 === 99 === The purpose of this study is to study the infrared irradiation effect on the gene expression of Arabidopsis thaliana and the PEP activity in photosynthesis. The narrow bandwidth plasmonic thermal emitter with specific emission waveband were designed and used to irradiate Arabidopsis for 72 hours, in order to identify the specific wavelength that induces the gene expression the most, that is, the NPQ4 and PSAK genes. Infrared emitters used in this study are fabricated based on the principle of surface plasmon. The purpose of the second experiment is to understand the effect of narrow band infrared irradiation on PEP-carboxylase activity in photosynthesis. The heat is generated by sending electric current to the molybdenum film on silicon substrate. The infrared source can be achieved by heating the triple layer structure which consists of a SiO2 layer between two Ag films on the molybdenum film. The top Ag layer is perforated by periodic hole array, and the emission wavelength can be altered by changing the lattice constant and diameter of the hole arrays. By RT-PCR analysis, it is found that the gene PSAK responds the most after illumination by PTE with peak wavelength at 4.5μm, and the gene NPQ4 exhibits the largest response by PTE with peak wavelength at 4.2μm. The experiments indicate that the PEPC activity can be enhanced by about 30~50% after infrared illumination, and the larger emission intensity would cause larger increase of activity. The PTE with specific peak wavelength at 4.3, 5.0, and 5.9 μm significantly enhances the activity. In general, after infrared illumination under different wavelengths, the PEPC activity can be enhanced. It implies that IR illumination can improve the carbon fixation process on the plants of C4 and CAM, and promote the efficiency of photosynthesis.
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