| Summary: | 碩士 === 國立臺灣大學 === 生化科技學系 === 99 === Obesity is associated with high risk for developing cardiovascular disease, metabolic syndrome and diabetes. In this study, we used the 3T3-L1 cell line as an in vitro model to examine the effects of Lactobacillus paracasei subsp. paracasei NTU 101, Lactobacillus plantarum NTU 102 and its’ fermented soy skim milk supernatant on proliferation and differentiation in preadipocyte as well as lipolysis and heparin-releasable lipoprotein lipase activity in mature adipocyte. Furthermore, we investigated the anti-obesity effects of NTU 101, NTU 102 and its fermented soy skim milk (SM101 and SM102) supplemented with high-fat diet on rats for five weeks as an in vivo animal model. As results in cellular experiment, NTU 101, NTU 102 and its’ fermented soy skim milk supernatant inhibited the proliferation and differentiation of 3T3-L1 preadipocyte, the inhibitory effects of NTU 101 and NTU 102 were better than its’ fermented products, the repression ratio was 26.8% and 53.1%, respectively. NTU 101 and NTU 102 fermented soy milk supernatant also significantly induced lipolysis and inhibited heparin-releasable LPL activity in
adipocyte, the elevation and inhibition ratio of NTU 101 fermented products were 26.4% and 30.4%, respectively. In animal experiment, high-fat diet supplemented with NTU 101, NTU 102, SM101 and SM102 can significantly decrease feed
efficiency, body weight and body fat pad weight of rats, the decrease ratio in SM101 was 53.2%, 49.7% and 55.9%, respectively. Moreover, fermented soy skim milk not
only significantly induced lipolysis and inhibited heparin-releasable LPL activity in adipocyte, but also reduced average radius of adipocyte and increased the numbers of
small size adipocyte. On the other hand, supplemented with NTU 101, NTU 102, SM101 and SM102 decreased serum lipidic parameters, proinflammatory cytokines and endotoxin in rats. These results revealed NTU 101, NTU 102 and its’ fermented soy skim milk may have the ability to inhibit inflammation induced by high-fat diet and reduce or prevent of body fat accumulation.
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