Crosslinking chitosan with PEG for wound dressing development study

• Main Authors: Yi-Ting Lai, 賴奕廷
• Other Authors: Kuo-Huang Hsieh
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/29808482447599437804

碩士 === 國立臺灣大學 === 化學工程學研究所 === 99 === Chitosan has outstanding biological properties, including biocompatible, biodegradable and antibacterial ability. Moreover, chitosan has hemostatic ability for promoting blood to coagulate together. However, the applications of chitosan are limited for its low mechanical properties. Chitosan existing in acid environment becomes a cationic polyelectrolyte due to its amine group, it can easily form polyelectrolyte complexes with other anionic polyelectrolytes and growth factors, or form crosslink structure with carboxylic group by composing amide bond. Through this strategy, the properties of chitosan can be modified and enhanced. In this research, we studied the properties of materials made by cross-linked chitosan with different poly(ethylene glycol)(PEG) molecular weight and various PEG to chitosan ratio. The physicochemical characterization and biological properties were studied for the PEG crosslinked chitosan films. Furthermore, an in vivo animal test using Male Balb/c mice showed rather good wound healing effect of PEG crosslinked chitosan film in the full-thickness skin defect model. From the histological examination, chitosan could suppress the infiltration of inflammatory cells and accelerate fibroblast proliferation while the PEG could enhance epithelial migration. Thus, PEG crosslinked chitosan films can be a potential wound dressing with excellent forming and enhanced wound healing.