| Summary: | 碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 99 === Increasing number of studies revealed that endogenous peptides are involved in various aspects of plant development and defense response. Most of the signaling peptides are known to be perceived by leucine-rich repeat receptor-like kinases (LRR-RLKs) in plants. Compared with other organism, plant has more numbers of LRR-RLKs. It is predicted that the plant have much greater number of bioactive peptide involved in the cell-cell communication. Currently, only a limited number of bioactive peptides were determined since 1991, while the first signal peptide, systemin, was discovered. The challenge for the discovery of plant peptide hormone is because of the low concentrations (nanomolar) of endogenous peptides, sample complexity and the lack of comprehensive analytical platform for plant signaling peptides.
Differential dimethyl labeling of N-termini peptides with formaldehyde and formaldehyde-13C, d2 combined with LC-MS/MS have been used for quantitation of endogenous peptides. In this study, we demonstrated this method can be used to label and quantify endogenous peptides in tomato leaves. With the use of this platform, fourty peptides can be identified and quantified. One of the peptides related to pathogen tolerance expressed in wounded leaves was studied and evaluated for the bioactivity. This efficient comprehensive analytical platform for the study of plant peptide signaling approach can help us to quantify bioactive peptides, which might be involved in biotic or abiotic stresses, pathogen defenses and developmental regulation in plants.
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