Study of viral infection mechanisms and inhibition ability of antiviral drugs using microcantilever sensors

• Main Authors: Shue-Hiu Luo, 羅淑慧
• Other Authors: Chih-Wei Wu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/24283130484843591528

碩士 === 國立臺灣海洋大學 === 機械與機電工程學系 === 99 === 　　Taiwan, located in the subtropical region, is one of the major epidemic areas of Japanese encephalitis, dengue fever and enterovirus. Yet up until now there is still no effective drug or vaccine that can truly prevent the infectious diseases mentioned above. Moreover, there are concerns regarding the side effects of the vaccines proved to be effective. Thus, it is an urgent priority to understand the infection process of the viruses in order to develop effective antiviral drugs or vaccines. 　　In the research, the micro-electro-mechanical systems were successfully integrated and with the combination of micro-cantilever sensor structure, micro-channel system, optical measurement system and hydrogel, a micro bio-chip was produced. The micro bio-chip can be used to test the infection process of a virus and evaluate the inhibiting effect of an antibody or an antiviral drug. The goals of rapid viral infection process detection and drug screening were achieved. The micro bio-chip successfully recorded complete infection processes of different viruses and different multiplicities of infection. It was found Japanese encephalitis had obvious penetration, replication and separation stages. Furthermore, dengue fever virus, which belongs to the same family of viruses as Japanese encephalitis, had a similar infection process. However, in terms of enterovirus 71 that belongs to a different family of viruses, it had a completely different infection process. In view of the detection results, for each of the virus mentioned above, the effective specific antibody and the non-effective non-specific antibody were respectively injected on the micro bio-chips along with the virus. The treatment effects of the antibodies were verified within a few hours. The tedious work and the time wasted in traditional immunoassay were avoided and there was no need for complicated animal experiments. In addition, the antiviral drug, algae extract, was screened in the research. It was proved the extract indeed had the capability to fight against dengue fever virus. The experiment result described above proved the micro bio-chip successfully reached to the goal of drug screening.