| Summary: | 博士 === 國立臺灣海洋大學 === 電機工程學系 === 99 === The main objective of this dissertation is to present a design of the power source for gene gun based on the principle of electromagnetic energy con-version. After the energy is stored in the capacitor, the energy discharged immediately toward the electromagnetic device of gene gun for providing micro-projectiles with electromagnetic force to generate the desired impact force to the T-type bullet, and the T-type bullet is then used to provide a speed to gold or tungsten particles so as to pass the cell wall of cell at the sample. Simultaneously, the outside gen follows the gold or tungsten parti-cles also passes to the cell or a tissue.
Based on the high pressure gas type gene gun, a hardware structure of electromagnetic gene gun as well as a main circuit and a control circuit are designed for one-stage electromagnetic gene gun to achieve the coupling between the electromagnetic device and the energy storage capacitor. The control circuit comprises a dc power supply circuit, an energy compensation circuit, a single chip control circuit, an optical-electric interface circuit, an energy storage capacitor charge/discharge circuit, an electromagnetic device, a zero voltage cross control circuit, a power supply control circuit, and a voltage comparator circuit. In addition, the control circuit is simulated by the PSpice software.
An electromagnetic gene gun is first implemented and tested under dif-ferent capacitance values to verify the feasibility. Experimental results re-veal that the gene gun is capable of providing a wide range of impact force. Furthermore, in order to reduce the size and increase the impact force, a two-stage electromagnetic control strategy is developed, in which a single chip is utilized to control the discharge time of dual capacitors for enhanc-ing the impact force. Comparative study of one-stage gene gun with two-stage gene gun shows that the two-stage gene gun can generate higher pressure than the traditional gene gun. In addition, according to the exper-imental results, with the same capacitance, the proposed gene gun can gen-erate higher impact force with smaller size and lower cost, and the validity and feasibility of the proposed electromagnetically powered gene gun is thus verified.
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