| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 99 === Moray eels are the commercially important fish species in Taiwan. In this study, acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted and characterized from the skins of moray eel (Gymnothorax favagineus) and milkfish (Chanos chanos). The results indicated that the yield of collagen extracted from moray eel and milkfish skins were approximately 43% and 51% on the basis of dry weight. According to the electrophoretic patterns, both ASC and PSC of moray eel and milkfish skins were belong to the type I collagen. The imino acid content of the collagen from moray eel and milkfish skins was lower than those of mammalian’s collagens, and the denaturation temperature were only 26℃ and 28℃, respectively. The ultraviolet (UV) absorption spectrum of both fish ASC and PSC showed that the distinct absorption was at 220-230 nm. In different concentrations of NaCl and pH, the solubility of both fish’s PSC were better than ASC. Results revealed that the characterizations of moray eel collagen was better than milkfish collagen.
Furthermore, collagen and gelatin of moray eel, milkfish and porcine skins were separately hydrolyzed by -chymotrypsin, trypsin and pepsin. The -chymotrypsin and trypsin hydrolysates of collagen and gelatin of moray eel and milkfish skins had the highest activity on ferrous ion chelating (90%). Both of the pepsin hydrolysates had the highest activity on DPPH radical scavenging (75%). The trypsin hydrolysates of collagen of moray eel and milkfish skins had the highest activity on ABTS+ scavenging (95%).
The inhibitory activity on angiotensin-I converting enzyme (ACE) of enzymatic hydrolysates from moray eel, milkfish and porcine skins were also investigated. The -chymotrypsin hydrolysates of collagen and gelatin of moray eel, milkfish and porcine skins had higher effect on ACE inhibiton (90%). All hydrolysates of collagen and gelatin did not showed cytotoxicity. The results suggest that the skin collagens of moray eel and milkfish possess powerful antioxidant activities and ACE inhibiton.
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