Study of the protective effects of Chlorella

• Main Author: 林睫侖
• Other Authors: Chang-Jer Wu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/28414569885065437711

碩士 === 國立臺灣海洋大學 === 食品科學系 === 99 === This study investigated the hot water extration of Chlorella pyrenoidosa (Chlorella pyrenoidosa extract, CpE) for inhibiting influenza A virus infection on in vitro and in vivo model. In vitro cell aaay, the sequence of CpE treatment is according to the adsorption step of virus infection; then divide into before virus adsorption (pre-treatment) and after virus adsorption (post-treatment). We use cell viability assay (MTS assay), cytopathic effect (CPE) assay, plaque assay, and viral RNA expression (real-time PCR) to study the protective effects of CpE. The beeter inhibition effect of CpE on influenza virus infection is during pre-treatment stage, and CpE-2 has the best protective effect. The concentration of CpE-1 and CpE-2 at more than 125 g/ml, the anti-hemagglutination ability of CpE has 2 HAU (hemagglutinating unit). CpE directly reacted the virus particles (virucidal) can effectively inhibit the plaque formation, but CpE directly reacted cell (cell-treatment) has no significant inhibitory effect. In virucidal treatment, 5 and 10 mg/ml of CpE-2 show more than 95% of inhibition effect. Above all, the inhibition mechanism of influenza virus infection of CpE is directly affecting on the adsorption of influenza virus to host cell. In BALB/c mice animal model, the 4 weeks-old mice infecte with lethal doses influenza virus and the mortality rate is 100%. The mice feeded CpE-2 for a week and CpE-2 direct treated with virus (virucide) before infection, the mortality rate of these mice are effectively reduced. RNA expression of mouse lung were detected by real-time PCR, influenza virus H1N1-NP, and inflammational cytokine: TNF-a, IL-1b, 2 IL-6, and antiviral cytokine: IFN-g, IL-12 in CpE-2 treated group were lower than virus alone group, histological also showed less infiltration of nertuophils, indicating CpE-2 treated can reduced virus infection and inflammation of lung. In summary, the Chlorella pyrenoidosa extract show a protective effect against influenza virus infection on both in vitro cell and animal experiment.