| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 99 === This study aimed to investigate Gelidium on carbonhydrate and lipid metabolism in rats with high fructose diet (57.1% w/w). Five weeks old Male Sprague-Dawley (SD) rats were fed high fructose diet to induce glucose intolerance and hyperlipidmia. The experiment divided into five groups: (1) control diet group, (2) control diet + Gelidium group, (3) high fructose diet group, (4) improvement group, and (5) prophylactic group. Experiment diets and drinking water were available ad libitum. After 12 weeks, the glucose intolerance and hyperlipidemia were determined by oral glucose tolerance test (such as HOMA-IR and concentration of plasma insulin), and blood plasma test (including total cholesterol, plasma triglyceride, and LDL+VLDL-C), respectively. Subsequently, rats with glucose intolerance and hyperlipidemia were evenly assigned to group 3 and 4 by their body weight and blood sugar levels. Each group of rats was fed experiment diet for eleven weeks.
Upon high fructose diet fed of SD rats, Gelidium supplement attenuated hyperlipidemia and glucose intolerance, as well as plasma TNF-a, fecal dry/wet weight and fecal lipid in both of improvement and prophylactic groups. Moreover, high fructose diet-activated liver lipogenesis enzymes (such as fatty acid synthase and acetyl-CoA carboxylase) and sterol regulatory element-binding proteins (including Srebp1 and Srebp2) were down-regulated in improvement and prophylactic groups. Besides, high fructose diet-upregulated the expression of Lpin1 and Lpin2 (fatty liver dystrophy protein), as well as the expression of Irs1 (insulin receptor substrate 1) in rat liver were attaneuated in improvement and prophylactic groups. Additionally, Gelidium suppressed food intake, plasma glucose, TC/HDL ratio, hepatic total cholesterol, glucose-6-phosphotase activity, leptin, plasma/hepatic TBARS, and AST activity; as well as increased HDL/(LDL-C+VLDL-C) ratio in prophylactic group. The high fructose diet upregulate the gene expression levels of Lipin1, Lipin2, and Irs1 in rat liver, These results suggested that Gelidium by may abate high fructose diet-induced glucose intolerance and hyperlipidemia the declining of carbohydrate absorbation at small intestine and hepatic lipogenesis of SD rats.
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