| Summary: | 碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 99 === The objective of this study was to investigate the pantothenic acid metabolism in hybrid tilapia (Oreochromis niloticus × O. aureus) with a modified cannulated urine collector through the observations on the 24-hour changes of plasma and urine concentration of pantothenic acid and Coenzyme A at various sampling time points as well as the 24-hour organ accumulation of these two metabolites after the initiation of exogenous pantothenic acid input either by oral administration or by caudal vein injection.
Hybrid tilapia averaged weight (900 ± 50)g of fish were used to observe the 24-hour time course changes of the plasma and urine pantothenic acid concentration after fish either orally forced fed diet containing 0, 5, 10, 20, 40, 80 mg/kg pantothenate or injected through caudal vein 5 ml of 0, 0.1, 0.3, 0.5 and 1 ppm pantothenate solution.
Plasma pantothenate level significantly elevated 2nd hour after the initiation of the experiment, then peaked at the 6th to 8th hour on all the treatment groups of fish orally forced fed diets containing various levels of pantothenic acid than that of the control group.
Pantothenic acid level in the plasma peaked at 2nd hour on all the treatment groups after fish injected different levels of pantothenate.
The emission value of pantothenic acid in urine increased according to the increasing concentration in diet or injection solution, but the increased percentage was negligible (less than 0.15%). The time course reduction of the plasma pantothenate led to the liver and muscle pantothenate accumulation.
The unknown percentage of pantothenate was around 20% in the 5 ppm dietary treatment which seemed parallel to the unknown percentage values of all the treatments in the injection groups. The abrupt increase from 20% (5ppm, dietary level) more than 50% of the pantothenate unknown percentage reflected by the fish received dietary level over 10 ppm implied the absorption rate of pantothenate in small intestine had reached a maximum speed. Significant increase of the 24-hour accumulation of pantothenate in the liver and muscle of hybrid tilapia had been observed at an dietary level of 10 ppm led the author to conclude that the optimal dietary level must fall between 5-10 ppm.
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