Imaging and analyzing the surface properties of purple membrane by AFM

• Main Authors: Lai,Yu-Cheng, 賴昱呈
• Other Authors: Fu,Tsu-Yi
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/52927198664600778103

碩士 === 國立臺灣師範大學 === 物理學系 === 99 === Purple membranes (PM) are the two-dimensional structure composed of Bacteriorhodopsin (BR) and many type's lipids. When PM is lighted, the proton transfers from cytoplasmic (CP) to extracellular (EC). Thus, the proton concentration gradient will be formed between CP and EC and then the photoelectric current is generated.CP and EC surfaces have different characteristics because they have different compositions. The observations were made by AFM.The fixed direction of PM adsorption on the substrate can be controlled by different processes. We found that the adhesion force of EC surface is slightly stronger than CP surface by Force-distance Curve analysis. The salt concentration also was found to affect the adhesion force. The salt concentration is higher, the adhesion force is stronger.