| Summary: | 碩士 === 國立臺南大學 === 體育學系碩士班 === 99 === Purpose：This study investigated the effect of endurance training and calorie restriction on skeletal muscle mass and energy metabolism in female rats for 8 weeks. Method：48 female Wistar rats（7 weeks）were randomly divided into four groups（n＝12 in each group）：low intensity (70% VO2max) endurance training（LIT）, high intensity (85% VO2max) endurance training（HIT）, control group（CON） and diet control group（DCON）. Exercise groups received treadmill training for 8 weeks：60 minutes a day and 5 days a week. For calorie restriction, DCON group were daily fed 80%-90% of exercise groups. Weights were recorded twice a week. Measured muscle weight and assayed activity of citrate Synthase (CS), 3-hydroxyacyl-CoA dehydrogenase (β-HAD), lactate dehydrogenase (LDH) and phosphofructokinase (PFK) after experiment for each group. Recorded data was analyzed by One-way analysis of variance (ANOVA) to compare weight, muscle weight and enzyme activity. Results：DCON group was significantly lower than HIT group, LIT group and CON group（p<.05）after 2th weeks. For muscle weight, Soleus (SOL) weight of DCON group was significantly lower than other 3 groups（p<.05）；Extensor digitorum longus (EDL) weight of DCON group was significantly lower than LIT and HIT groups. On the part of skeletal muscle energy metabolism enzyme, there was no significant effect in CS, β-HAD, LDH and PFK activity of SOL, and no significant effect in CS, β-HAD and PFK activity of EDL, either. DCON group is higher than HIT group（p<.05）in LDH activity. Conclusion：Calorie restriction lower weight in female rats. In addition, there wasn’t significant effect in endurance training to increase energy metabolism enzyme activity in skeletal muscle. However, calorie restriction increased LDH activity in EDL.
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